Exposing E. Coli
|University of Texas at Austin molecular biology doctoral student Jeff Tabor holds a bacteria-produced photo of an enlarged E. coli bacterium a "self portrait." Credit: Marsha Miller/The University of Texas at Austin|
Using Petri dishes full of genetically engineered E. coli instead of photo paper, students at The University of Texas at Austin and UCSF successfully created the first-ever bacterial photographs. Their work is published in this week’s issue of Nature (Nov. 24, 2005), which is devoted entirely to the emerging field of synthetic biology.
The students produced the innovative bacterial images and a bacterial camera as part of MIT’s intercollegiate Genetically Engineered Machine (iGEM) competition, which encourages students to build simple biological machines.
"The goal of the contest was to build bacteria that could do very simple computing," says Dr. Edward Marcotte, one of the students’ faculty advisers and associate professor of biochemistry at The University of Texas at Austin. "This is a great example of the emergent field of synthetic biology–using principles of engineering in biology." "We’re making bacteria that are all independently functioning computers and we can get them to do large-scale, complex computations like make images or create circuits," says Jeff Tabor, a doctoral student at the Institute for Cell and Molecular Biology in Austin. The students produced ghostlike, living photos of many things, including themselves, their advisers and The University of Texas Tower.
The bacterial photos were created by projecting light on "biological film"–billions of genetically engineered E. coli growing in dishes of agar, a standard jello-like growth medium for bacteria. Like pixels on a computer screen switching between white and black, each bacterium either produced black pigment or didn’t, based on whether it was growing in a light or dark place in the dish. The resulting images are a collection of all the bacteria responding to the pattern of light.
E. coli are found naturally in the dark confines of the human gut and wouldn’t normally sense light, so the students had to engineer the unicellular machines to work as a photo-capturing surface. UCSF biophysics graduate student Anselm Levskaya and his adviser, Dr. Chris Voigt, first engineered the bacteria to sense light by adding a light receptor protein from a photosynthetic blue-green algae to the E. coli cell surface. They hooked the light receptor up to a sensor in E. coli that normally senses salt concentration. Instead of sensing salt, the bacteria could sense light.
|Members of The University of Texas at Austin team. Pictured here from left to right are Laura Lavery, senior in biochemistry; Jeff Tabor, doctoral student in molecular biology; Matt Levy, postdoctoral fellow in the Institute for Cellular and Molecular Biology (ICMB); Eric Davidson, graduate student in ICMB; and Aaron Chevalier, senior in physics. Credit: Marsha Miller/The University of Texas at Austin|
The light receptor was then connected to a system in the bacteria that makes pigments. When light strikes the new receptor, it turns off a gene that ultimately controls the production of a colored compound in the bacteria. The Texas students, including Tabor and Aaron Chevalier, realized that after optimizing the pigments and agar growth media, these bacteria could be used to convert light images shined onto the bacteria into biochemical prints. To create the photographs, the Texas students used a unique light projector largely designed and built by Chevalier, a physics undergraduate.
The device projects the pattern of light–like an image of one of the Texas students’ co-advisers, Dr. Andy "Escherichia" Ellington–onto the dish of bacteria growing at body temperature in an incubator. After about 12-15 hours of exposure (the time it takes for a bacterial population to grow and fill the Petri dish), the light projector is removed. What’s left is a living photograph.
Bacteria in the lighted regions of the Petri dish don’t produce the pigment and appear light. Those in the dark regions produce pigment and appear dark.
The biological technologies these students are building could be applied in a variety of ways beyond making photos, says Marcotte. For example, he says the techniques could one day be used to build different tissues based on patterns of light or make bacteria that can produce structures useful in medical treatments.
The students are already busy on their next innovation–bacteria that can find and create a line around the edges of an image, a process that requires the bacteria to communicate with each other. They’re also working on experiments using a laser to turn on and off single cells, which would give them great control.
"If we can hit the cell with a laser, we can manipulate their biology without needles or syringes," says Tabor. "We just turn it on or off with light."